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Home > Cats > 6 Homemade Enzyme Cleaner for Cat Urine Recipes That Work!

6 Homemade Enzyme Cleaner for Cat Urine Recipes That Work!

Cat looking on as human spray cleans carpet

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Reviewed & Fact-Checked By

Dr. Lorna Whittemore

Veterinarian, MRCVS

The information is current and up-to-date in accordance with the latest veterinarian research.

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Cleaning cat urine accidents can be tricky. Even when the stains have been cleaned, the odor can still linger. The best thing to do when it comes to cat pee is to remove the stain as soon as possible. The quicker you can start destroying the odors, the faster your home can get back to smelling fresh. There is the dual problem of removing the stain and odor you can smell and that which your feline friend can even after it looks clean to you.  If not cleaned properly they may be drawn to use the same area again as a toilet.

To do the job right, you need to have the right cleaners on hand.

But if you don’t feel like buying an enzyme cleaner, no problem! We gathered easy DIY recipes that you can make using common household ingredients to get rid of cat urine unpleasantness right away.

First, we look at homemade cleaners that are simple to put together and immediately ready to use. Then, there’s an enzyme cleaner recipe recommended by vets for tricky urine stains. Last is a carpet refresher recipe. It is important that you test any recipe on an inconspicuous small patch of carpet to ensure no lifting of color or damage, especially wool carpets. With all the recipes, start by soaking up as much urine as possible with plain paper towels, using a pressing rather than rubbing action.

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The 6 Homemade Enzyme Cleaner for Cat Urine Recipes

1. Hydrogen Peroxide Cat Urine Cleaner

  • ¾ cup hydrogen peroxide
  • 1 teaspoon dish soap
  • Baking soda

Cover the cat pee stain liberally with baking soda. Allow the baking soda to sit for up to 2 hours. While it’s sitting, combine the hydrogen peroxide and dish soap in a small bowl or glass.

Pour the mixture over the baking soda, and let it fully absorb, working the solution down into the carpet. Blot up any excess liquid with paper towels.

When the stain is dry, vacuum up the baking soda.


2. White Vinegar Cat Urine Cleaner

white vinegar on the wooden table top
Image By: focal point, Shutterstock
  • Baking soda
  • 4 tablespoons hydrogen peroxide
  • 1 teaspoon dish soap
  • White vinegar

Pour baking soda over the affected area. After 10 minutes, pour vinegar over the baking soda. Blot up any excess liquid with paper towels, and let the mixture sit for 1 minute.

Mix hydrogen peroxide and dish soap in a bowl, and scrub it into the stain over the vinegar and baking soda. Use an old toothbrush or cloth to work the mixture into the area.

Blot the stain with paper towels.


3. Apple Cider Vinegar Cat Urine Cleaner

  • ½ cup warm water
  • ½ cup apple cider vinegar

Apple cider vinegar is made of acidic ingredients that eliminate odor-causing bacteria and neutralize alkaline components of the urine.

Mix water and apple cider vinegar in a bowl, and pour the solution over the affected area. Let it sit for 5 minutes. Blot up any excess liquid with paper towels.


4. Hydrogen Peroxide and Water Cat Urine Cleaner

Hydrogen Peroxide
Image By: pedphoto36pm, Shutterstock

This recipe is good to make and keep on hand in a spray bottle for accidents around the litter box or on hardwood floors and walls.

  • ½ cup hydrogen peroxide
  • 1 cup water

Add ingredients to a spray bottle. When cat pee is on hard surfaces around your home, wipe up the pee with paper towels. Then use this cleaner to spray the area to combat any odors. Dry with paper towels.


5. Enzyme Cat Urine Cleaner – Vet approved

  • Biological washing powder
  • Hot water
  • Bicarbonate of soda
  • Surgical Spirit (clear)
  • Spray bottle
  • Paper towels

Using the Enzyme Cleaner

  • Step 1 – Make a mix of biological washing powder and a small amount of very hot water to dissolve it. The proteases in biological washing powder help to break down the biological components of cat urine. This helps to get rid of the smells that you, and your cat can smell.
  • Step 2 – Add warm water to the powder mix until you have a solution that is 1 part biological washing powder to 9 parts warm water, a 10% solution.
  • Step 3 – Soak the urine spot in the solution and leave for 30-60 minutes to work.
  • Step 4 – Blot up the remaining liquid with a plain paper towel. Rinse the area with clean water and repeat soaking up with a paper towel until the detergent is gone.
  • Step 5 – Liberally apply bicarbonate of soda powder to draw out any remaining moisture and leave for 12 hours.  Use a vacuum cleaner to lift off the dried powder.
  • Step 6 – Put surgical spirit into a spray bottle and spray over the affected area and leave to dry.  The surgical spirit breaks down any remaining fats from the urine and is an important last step.

If you're trying to find an enzyme cleaner that does it all, we highly recommend our favorite cleaner, the Hepper Advanced Bio-Enzyme Pet Stain & Odor Eliminator Spray.

Hepper Advanced Bio-Enzyme Pet Stain & Odor Eliminator Spray
  • ADVANCED ENZYMATIC CLEANER - Penetrates the most stubborn smells and stains at the deepest molecular...
  • FOR ANY MESS, ON ANY SURFACE - This pet odor eliminator cleans your carpets, floors, furniture,...
  • FRESH, NATURAL ODOR - Our unique formulation doesn't rely on dangerous or unpleasant chemical...

It permanently removes the very worst stains and smells you can imagine and makes clean up a breeze. There's even a 100% satisfaction guarantee! Click here to order a bottle today.

At Pet Keen, we’ve admired Hepper for many years, and decided to take a controlling ownership interest so that we could benefit from the outstanding products of this cool cat company!

6. Carpet Refresher

spraying carpet cleaner on the carpet
Image By: Syda Productions, Shutterstock
  • 1 cup baking soda
  • ¼ cup dried crushed lavender

Mix the ingredients. Sprinkle the mixture over your carpet. Let it sit for a minimum of 20 minutes, and then vacuum.


For Cleaning Clothes

If your favorite shirt is the unlucky recipient of a cat urine accident, rinse out as much of the urine as possible without soaking the whole garment. Then run the clothing through the washing machine using biological washing powder.

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Final Thoughts

DIY cleaners may take a while to put together, but the reward makes it worthwhile. These effective cleaners can get rid of urine stains and odors without any of the harsh chemicals that may be dangerous to pets and children.  Do not use ammonia containing products as they draw cats to the area because it smells like urine to them.

These homemade cat urine cleaner recipes are simple to prepare and budget-friendly, but if you prefer the convenience of a shop-bought product then Simple Solution offers a great range. Remember to always test a small area of your carpet before treating the whole stain and if your cat is regularly urinating out of the litter tray be sure to get them checked out by their vet.


Featured Image Credit by: Kristi Blokhin, Shutterstock

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